Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
| Name |
Number |
Comment |
| crRNA |
|
|
Topology
Parts
| Name |
Number |
Comment |
| pLac |
|
promotor induced by IPTG |
| IPTG |
|
|
| cI |
|
cI protein |
| pLux/cI |
|
pLux promotor is repressed by cI protein |
| Cas3 |
|
Cas3 nuclease - cleaves DNA |
| Cd |
|
Cascade complex - guides crRNA to its target gene |
| crRNA |
|
CRISPR-RNA - complementary RNA to target gene (here DNA Pol III and RNA Pol) |
| LuxR+LuxI |
|
LuxI protein (homoserine lactone) forms a complex with LuxR which bind to the pLux promotor and activates it |